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DyNAzyme™ II Recombinant DNA Polymerase Kit

 

Complete kit with a control protocol for standard PCR

 
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Description

DyNAzyme™ II DNA polymerase kit is available in two sizes. The small kit (F-551S) contains all necessary reagents for 100 amplifications of 50 µl each and the large kit (F-551L) contains reagents for 500 amplifications. The kits also contain control template ( DNA) and primers for 40 control reactions. A ready to use DNA Size Standard ( DNA-Hind III and řX174 DNA- Hae III digest) sufficient for 40 lanes is also included in the kits.

Kits include a control protocol for PCR.

 

To check the performance of the PCR reaction, an amplification of 500 bp of l DNA can be made with the control template and the control primers included in the DyNAzyme™ PCR kits. The complete control protocol is included in the kits. The picture shows the result of the control reaction:
Lane 1 DNA size standard (F-303 SD)
Lane 2 Amplified 500 bp product of control PCR
Lane 3 DNA size standard (F-303 SD)

 

 
DyNAzyme™ II DNA Polymerase kit components

Component

Concentration

F-551S

F-551L

DyNAzyme™ II Recombinant Enzyme

2 U/µl

100 U

500 U

dNTP Mix

10 mM/each

100 µl

500 µl

Optimized DyNAzyme™ buffer

1 x 1.5 ml

2 x 1.5 ml

Mg2+-free DyNAzyme™ buffer

1 x 1.5 ml

2 x 1.5 ml

MgCl2-solution

50 mM

1 x 1.5 ml

1 x 1.5 ml

Control Template ( DNA)

0.5 ng/µl

40 µl

40 µl

500 bp primers

25 µM/each

40 µl

40 µl

       

DNA Size Standard

     

( DNA Hind III + řX174 DNA Hae III digests)

50 ng/µl of each

400 µl

400 µl

Storage

Stable for at least 1 year. Recommended storage temperature -20 °C.

 

 


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