DyNAzyme I Kit

DyNAzyme™ I Native DNA Polymerase Kit

Complete kit with a control protocol for standard PCR

Description

The DyNAzyme™ I DNA polymerase kit is available in two sizes. The small kit (F-550S) contains all necessary reagents for 100 amplifications of 50 µl each and the large kit (F-550L) contains reagents for 500 amplifications. The kit also contains control template ( DNA) and primers for 40 control reactions. A ready to use DNA Size Standard ( DNA -Hind III and øX174 DNA- Hae III digest) sufficient for 40 lanes is also included in the kit.

Storage

Stable for at least 1 year. Recommended storage temperature -20 °C

DyNAzyme™ I DNA Polymerase Kit
Component	Concentration	F-550S	F-550L
DyNAzyme™ I Native Enzyme	2 U/µl	100 U	00 U
dNTP Mix	10 mM/each	100 µl	500 µl
Mg²⁺-free DyNAzyme™ EXT buffer		1 x 1.5 ml	2 x 1.5 ml
Optimized DyNAzyme™ EXT buffer		1 x 1.5 ml	2 x 1.5 ml
MgCl₂-solution	50 mM	1 x 1.5 ml	1 x 1.5 ml
Control Template ( DNA)	0.5 ng/µl	40 µl	40 µl
500 bp primers	25 µM/each	40 µl	40 µl
DNA Size Standard ( DNA Hind III
+ øX174 DNA Hae III digests)	50 ng/µl of each	400 µl	400 µl

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