DyNAzyme™ –Buffers

Finnzymes provides five different DyNAzyme™ buffers and a bufferpack for DyNAzyme™ EXT. DyNAzyme™ DNA polymerases are provided with optimized 10 x buffer (1 x buffer contains 1.5 mM MgCl₂), or with Mg²⁺ -free 10 x buffer and MgCl₂ -solution. Detergent- free buffers doesn't include any Triton® X-100.

Mg²⁺ -free DyNAzyme™ buffer (10 x stock solution) and 50 mM MgCl₂ –solution

1 x Mg²⁺ –free buffer (10 x buffer is diluted 1:10) contains: 10 mM Tris-HCl (pH 8.8 at 25 °C), 50 mM KCl and 0.1 % Triton^® X-100  

Optimized DyNAzyme™ buffer (10 x stock solution)

x buffer contains: 10 mM Tris-HCl (pH 8.8 at 25 °C), 1.5 mM MgCl₂, 50 mM KCl and 0.1 % Triton^® X-100  

Optimized DyNAzyme™ EXT buffer (10 x stock solution) and 50 mM MgCl₂ –solution

1 x buffer contains: 50 mM Tris-HCl (pH 9.0 at 25 °C), 1.5 mM MgCl₂, 15 mM (NH₄)₂SO₄ and 0.1 % Triton^® X-100  

DyNAzyme™ EXT Bufferpack  

Optimized detergent-free DyNAzyme™ Buffer (10 x stock solution)

1 x buffer contains: 10 mM Tris-HCl (pH 8.8 at 25 °C), 1.5 mM MgCl₂, 50 mM KCl. 

Optimized detergent-free DyNAzyme™ EXT Buffer (10 x stock solution)

1 x buffer contains: 50 mM Tris-HCl (pH 9.0 at 25 °C), 1.5 mM MgCl₂, 15 mM (NH₄)₂SO₄.