Alpha Innotech

Superior Performance

Fluorescence, Chemiluminescence and Visible Imaging Systems

FluorChem™ Imaging System is designed with key advantages in real time Resolution, Sensitivity, Dynamic range and Performance setting the RSDP standard.

FluorChem™ Sample Fluorescent & Colorimetric Images

Multiplex Array

Fluorescein Gel

SYPRO®
Red Protein Gel

GFP Application

EtBr DNA Gel

Multiplex Gel

Coomassie Blue Gel

FluorChem™ Imaging System Chemiluminescent Macroarray Sample Images

	StarProfiler™ Array from Genemed Image of Star Profiler™ Array with CDP-Star generated with FluorChem™ Exposure time: 5 min
	Spotlight™ array from Clontech The Atlas™ Human 1.2 Array II visualized using chemiluminescent detection. Array hybridization was performed with biotinylated cDNA probes prepared from 2 micrograms of human brain poly A+ RNA or 2 microgram of human placenta poly A+ RNA. Hybridization and detection were performed using the SpotLight Hybridization & Detection Kit.
	GEArray™ from SuperArray, Inc.
	TranSignal Protein/DNA Array imaged with the FluorChem Imaging System

Key RSDP Features of FluorChem™ Imaging System

Resolution

	Megapixel CCD resolution.
	Spatial resolution zoom from 30-180 um.
	Proprietary algorithms for 3D image contour, image sharpening, and noise reduction for improved image resolution and quality.

Sensitivity

	High quantum efficiency for superior sensitivity with proprietary optical and sensor designs.
	Deep CCD peltier cooling facilitates multiple hour long exposures and exceptional image quality.
	F1.2 zoom optics maximize light transmission without optical distortions. Zoom optics are available in manual or motorized configurations with AlphaEaseFC™ software control.
	Optional adjustable tray is available for moving chemiluminescence samples closer to the CCD detector for improved sensitivity and spatial resolution with easy sample target sizes.
	ChemiGlow™ chemiluminescence substrate kit is an ideal addition to FluorChem™ as an optimized substrate for CCD imaging technology to generate femtogram sensitivity levels.

Dynamic Range

	0-4 O.D. measureable dynamic range with 16-bit file archiving format
	Automatic exposure algorithms in FluorChem™ AlphaEaseFC™ software easily ensures maximum image dynamic range.
	'Intelligent' AlphaEaseFC™ software AND ergonomic MultiImageFC™ Cabinet touch panel excitation sources, automatic filter wheel, and motorized optics controls for total ease of use in grayscale and multiplex imaging.

Performance

	Fast, easy, versatile, and powerful for comprehensive fluorescence and chemiluminescence applications AND basic gel documentation.
	No extra gel documentation camera modules are required.
	Real time image display in the Focus mode for easy sample positioning and optics adjustment.
	FluorChem™ AlphaEaseFC™ 'one button click' automatic enhancement and analysis for 1D lane analysis, 2D spot densitometry, M.W./Rf calculations, automatic colony and cell counting, microtiter plate analysis, macroarray analysis, object distance measurements, and gel scoring.
	Automatic lane/band detection and analysis with smiling corrections are easily done with a single button click!
	Capture grayscale or true color images with automatic RGB color image overlay feature.
	Total software automation with FluorChem AlphaEaseFC™ providing the unique MovieMode™ acquisition function making chemiluminescence multiple image capture a breeze.
	SpectraImager™ color microscopy camera add-on provides versatility in image acquisition to create a core imaging workstation for gel, membrane, plate, and microscopy imaging applications.

Northern blot with CSDP® label image for 5 min. with FluorChem™.	Northern blot with 20 min. exposure to film.	Southern blot with ECL® imaged for 5 min. with FluorChem™.	Southern blot with 5 min. exposure to film.
Western chemiluminescent blot imaged with FluorChem™. Faster than film!	Western chemiluminescent blot imaged with film.	Atlas™ array with CDPStar® imaged for 5 min. with FluorChem™.	Atlas™ array with 60 min. exposure to film.

Key Technical Specifications

Alpha Innotech provides up to 5 unique excitation sources to maximize sensitivity with all fluorescence and colorimetric imaging. Excitation is available in transillumination and epi-illumination formats in wavelengths ranging from 225-495nm for UV and ChromaLight™ Blue as well as 400-700nm for visible light imaging.

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